Release of 14C-serotonin during initial platelet changes induced by thrombin, collagen, or A23187
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چکیده
منابع مشابه
Release of platelet fibronectin (cold-insoluble globulin) from alpha granules induced by thrombin or collagen; lack of requirement for plasma fibronectin in ADP-induced platelet aggregation.
Platelets lysed with Triton X-100 contain 3.44 +/- 1.27 (SD) microgram of fibronectin (cold-insoluble globulin) per 10(9) platelets. Fibronectin was partially released from washed whole platelets by collagen or thrombin, and its release by collagen was inhibited by aspirin. Analysis of subcellular fractions obtained by density-gradient centrifugation of disrupted platelets indicated that fibron...
متن کاملMicrovesicle release is associated with extensive protein tyrosine dephosphorylation in platelets stimulated by A23187 or a mixture of thrombin and collagen.
Phosphatidylserine exposure and microvesicle release give rise to procoagulant activity during platelet activation. We have previously shown that whereas the Ca2+ ionophore A23187 and 2,5-di-(t-butyl)-1, 4-benzohydroquinone, a Ca2+-ATPase inhibitor, induce phosphatidylserine exposure, only the former triggers microvesicle release. We now report that microvesicle formation with ionophore A23187 ...
متن کاملInhibition and potentiation of platelet function by lysolecithin.
The effects of lysolecithin (LPC) on aggregation, serotonin release, shape, and lysis of rabbit, pig, or human platelets in platelet-rich plasma (PRP) or Tyrode albumin solution were examined during prolonged incubation. LPC added to citrated or heparinized PRP from humans or rabbits at a final concentration above 100 muM caused instantaneous inhibition of platelet aggregation induced by adenos...
متن کاملStudies on Thrombin-induced Platelet
The importance of morphologic alteration, clumping, and subsequent fusion of blood platelets in hemostasis and thrombosis is well known. The mechanisms underlying these platelet changes, often referred to as agglutination and viscous metamorphosis, are poorly understood. Since the early work of Wright and Minot (1), many studies have suggested that thrombin may be involved. Widely divergent con...
متن کاملPlatelet activation by collagen provides sustained release of anabolic cytokines.
BACKGROUND Platelet-rich plasma (PRP) has been increasingly used in sports medicine applications. Platelets are thought to release growth factors important in wound healing, including transforming growth factor (TGF-β1), platelet-derived growth factor (PDGF-AB), and vascular endothelial growth factor (VEGF). However, little is known about the effect of platelet activator choice on growth factor...
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ژورنال
عنوان ژورنال: Blood
سال: 1977
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v50.5.915.bloodjournal505915